effect of diets containing soybean meal or canola meal on anaerobic fungal population in rumen using quantitative competitive pcr.
نویسندگان
چکیده
the aim of this study was to evaluate the effect of substitution of soybean meal with canola meal and measure its effects on rumen anaerobic fungal population in lactation iranian of holstein cows. from d 5 to 56 postpartum, cows were fed diets that were isoenergetic containing soybean meal (sbm; n = 3) or canola meal (cm; n = 3). cows were housed in tie stalls and fed tmr two times a day. competitive pcr technique was used to evaluate quantitative difference of anaerobic fungal population within the rumen under the dietary groups. universal primers were used to amplify a specific region of the rrna locus from anaerobic rumen fungi. standard control dna was constructed for use in the competitive pcr and was shown to amplify under the same reaction condition and with the same amplification efficiency as the target dna. the relative intensities of pcr products were used to compare variety of fungal population under fed treatments. the signal intensity was quantified by imagej 1.29x and expressed in arbitrary units. the data was analyzed using the glm procedure of sas for a completely randomized design. the rumen fungal population was not impacted by diet but numerically was decreased in the cm compare with the sbm diet (34.79 and 35.12 ± 1.87 arbitrary units, respectively). the results of this study demonstrated that substituting soybean meal with canola meal in the early lactation cows had no apparent effect on the fungal population..
منابع مشابه
the comparison of rumen fungi quantification in the medium containing sunflower meal treated with formaldehyde and or sodium hydroxide by using quantitative competitive pcr
the method of quantitative competitive polymerase chain reaction (qc-pcr) was conducted to compare the number of rumen anaerobic fungi in pure culture of fungi containing high fat sunflower meal (165 g fat/kg dm) processed with formaldehyde and naoh. twenty one multiparous early lactating holstein cows (30±5 days of lactation) selected and fed experimental diets for 7 weaks. the diets were incl...
متن کاملEffect of Microbial Phytase in Soybean Meal Based Broiler Diets Containing Low Phosphorous
A six wk feeding experiment was conducted with d-old four hundred broiler chicks (Ven Cobb) to determine the effects of microbial phytase (Allzyme) supplementation in soybean meal based broiler diet containing low phosphorous. These birds were randomly divided into four dietary treatment groups of 100 broilers each. Each treatment group was further sub-divided into five replicates of 25 broiler...
متن کاملEffects of soybean meal or canola meal on milk production and methane emissions in lactating dairy cows fed grass silage-based diets.
This study evaluated the effects of soybean meal (SBM) and heat-moisture-treated canola meal (TCM) on milk production and methane emissions in dairy cows fed grass silage-based diets. Twenty-eight Swedish Red cows were used in a cyclic change-over experiment with 4 periods of 21 d and with treatments in 2 × 4 factorial arrangement (however, the control diet without supplementary protein was not...
متن کاملUsing Extruded-Expelled Soybean Meal in Swine Diets
Jason Woodworth, Graduate Research Assistant Extrusion processing is an effective means of improving the nutritional value of whole soybeans fed to swine. Recently, Insta-Pro International has combined extrusion technology with expelling, an oil extraction process, to produce an extruded-expelled soybean meal (Insta-Pro ExpressTM extruder/press system). The resulting soybean meal contains sligh...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
پژوهش های علوم دامی ایرانجلد ۱، شماره ۲، صفحات ۰-۰
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023